Technologies

The Gene Circuit Designer (GCD) is a comprehensive software tool for the design and simulation of synthetic gene circuits in living cells. It provides a user-friendly interface for creating and manipulating genetic circuits, as well as simulating their behavior under various conditions. The GCD is built on top of a robust mathematical framework that models gene expression, protein interactions, and other cellular processes.The GCD allows users to create genetic circuits using a library of pre-defined genetic components, including promoters, genes, and regulatory elements. Users can drag-and-drop these components onto a canvas to create a circuit diagram, which can then be simulated using a variety of numerical methods. The simulator takes into account various cellular processes, such as transcriptional regulation, translation, and protein degradation, allowing users to predict the behavior of their designed circuits.The GCD also includes a comprehensive database of genetic components, including their experimentally measured parameters, such as promoter strengths, gene expression levels, and protein-protein interaction affinities. This database allows users to select components with specific properties and incorporate them into their designs. Furthermore, the GCD provides tools for analyzing and optimizing circuit performance, including sensitivity analysis, parameter estimation, and circuit optimization.The GCD has been extensively validated using experimental data from various organisms, including bacteria, yeast, and mammalian cells. Its simulation results have been shown to accurately predict the behavior of synthetic gene circuits, allowing researchers to design and test novel circuits with high confidence. The GCD is a valuable tool for synthetic biologists, bioengineers, and researchers in related fields, enabling them to accelerate the design and construction of novel biological systems with specific functions.