Polymerase Chain Reaction (PCR) Technology is a widely used method in molecular biology to amplify specific DNA sequences. The process involves the use of an enzyme called Taq polymerase, which synthesizes new DNA strands by adding nucleotides to a template DNA. The PCR process involves three main stages: Denaturation: The DNA double helix is heated to separate it into two single strands. Annealing: Primers bind to their complementary DNA sequences on the single strands. Extension: The Taq polymerase reads the template DNA and adds nucleotides to synthesize new DNA strands.PCR Applications include: Genetic testing: Detection of genetic disorders and infectious diseases. Forensic analysis: DNA profiling for crime scene investigation. Research and development: Gene cloning, mutation analysis, and gene expression analysis.PCR Advantages include: High sensitivity: Amplification of rare DNA sequences. High specificity: Accurate detection of target DNA sequences. Rapid results: Quick turnaround time for DNA amplification.